WWTR1 antibody

Catalog No. ABIN7138224

The Rabbit Polyclonal anti-WWTR1 antibody has been validated for WB and ELISA. It is suitable to detect WWTR1 in samples from Human.

Quick Overview for WWTR1 antibody (ABIN7138224)

Target: WWTR1 (WW Domain Containing Transcription Regulator 1 (WWTR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WWTR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-WWTR1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Peptide sequence around aa.386~390 (V-E-S-A-L) derived from Human TAZ.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for WWTR1

Target: WWTR1 (WW Domain Containing Transcription Regulator 1 (WWTR1))

Alternative Name: WWTR1

Background:

Background: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.

Kanai F., Marignani P.A., Sarbassova D., Yagi R. EMBO J. 19:6778-6791(2000)
Lei Q.Y., Zhang H., Zhao B., Zha Z.Y. Mol. Cell. Biol. 28:2426-2436(2008)
Varelas X., Sakuma R., Samavarchi-Tehrani P., Peerani R. Nat. Cell Biol. 10:837-848(2008)
Di Palma T., D'Andrea B., Liguori G.L., Liguoro A. Exp. Cell Res. 315:162-175(2009)

Aliases: DKFZP586I1419 antibody, FLJ27004 antibody, FLJ45718 antibody, OTTHUMP00000215994 antibody, OTTHUMP00000215995 antibody, OTTHUMP00000215996 antibody, OTTHUMP00000216001 antibody, TAZ antibody, Transcriptional co activator with PDZ binding motif antibody, Transcriptional coactivator with PDZ binding motif antibody, Transcriptional coactivator with PDZ-binding motif antibody, WW domain containing transcription regulator 1 antibody, WW domain containing transcription regulator protein 1 antibody, WW domain-containing transcription regulator protein 1 antibody, WWTR 1 antibody, WWTR1 antibody, WWTR1_HUMAN antibody

UniProt: Q9GZV5

Pathways: Regulation of Lipid Metabolism by PPARalpha