SP1 antibody (pThr739)

Catalog No. ABIN7138879

The Rabbit Polyclonal anti-SP1 antibody (ABIN7138879) specifically detects SP1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for SP1 antibody (pThr739) (ABIN7138879)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SP1 Antibody

Binding Specificity: pThr739

Purpose: Phospho-SP1 (Thr739) Antibody

Cross-Reactivity: Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of threonine 739 (T-A-T(p)-P-S) derived from Human SP1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: SP1

Background:

Background: Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-a G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Thiesen HJ,et al.(1990)Nucleic Acids Res.18(11):3203-9.
Zutter MM,et al.(1997) Blood.90(2):678-89.
Lou Z,et al.(2005)Cancer Res.65(3):1007-17

Aliases: SP 1 antibody, SP1 antibody, Sp1 transcription factor antibody, SP1_HUMAN antibody, Specificity protein 1 antibody, Transcription factor Sp1 antibody, TSFP 1 antibody, TSFP1 antibody

UniProt: P08047

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction