African Cassava Mosaic Virus (ACMV) AC1 (AA 1-358) antibody (Biotin)

Catalog No. ABIN7140390

The Rabbit Polyclonal anti- antibody has been validated for ELISA. It is suitable to detect in samples from African cassava mosaic virus (ACMV).

Quick Overview for African Cassava Mosaic Virus (ACMV) AC1 (AA 1-358) antibody (Biotin) (ABIN7140390)

Target: African Cassava Mosaic Virus (ACMV) AC1

Reactivity: African cassava mosaic virus (ACMV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA

Product Details

Binding Specificity: AA 1-358

Virus Strain: isolate West Kenyan 844

Purpose: AC1 Antibody, Biotin conjugated

Cross-Reactivity: African cassava mosaic virus (ACMV)

Purification: Protein G purified

Purity: >95 %

Immunogen: Recombinant African cassava mosaic virus Replication-associated protein (1-358AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details

Target: African Cassava Mosaic Virus (ACMV) AC1

Background:

Background: Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5\'-TAATATTAC-3\' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5\'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3\'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity).

Aliases: AC1 antibody, AL1 antibody, Replication-associated protein antibody, Rep antibody, EC 2.7.7.- antibody, EC 3.1.21.- antibody, 40.4 kDa protein antibody, Protein AC1 antibody, Protein AL1 antibody

UniProt: P14982