AP2B1 antibody (AA 738-937)

Catalog No. ABIN7144363

Product Details anti-AP2B1 Antibody

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Binding Specificity: AA 738-937

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP2B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human AP-2 complex subunit beta protein (738-937AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for AP2B1

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Alternative Name: AP2B1 (AP2B1 Products)

Background:

Background: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins, at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly, clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.

Aliases: Adapter related protein complex 2 beta 1 subunit antibody, Adapter-related protein complex 2 beta subunit antibody, Adaptin, beta 2 (beta) antibody, Adaptor protein complex AP-2 subunit beta antibody, Adaptor related protein complex 2, beta 1 subunit antibody, ADTB2 antibody, AP-2 complex subunit beta antibody, AP105B antibody, AP2 BETA antibody, Ap2b1 antibody, AP2B1_HUMAN antibody, Beta adaptin antibody, Beta-2-adaptin antibody, Beta-adaptin antibody, Beta2 adaptin antibody, CLAPB1 antibody, Clathrin assembly protein complex 2 beta large chain antibody, Clathrin associated/assembly/adaptor protein, large, beta 1 antibody, DKFZp781K0743 antibody, Plasma membrane adaptor HA2/AP2 adaptin beta subunit antibody

UniProt: P63010

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation