AP2B1 antibody (Middle Region)

Catalog No. ABIN2785454

There is 1 publication for this product available. The Rabbit Polyclonal anti-AP2B1 antibody is suitable to detect AP2B1 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for AP2B1 antibody (Middle Region) (ABIN2785454)

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP2B1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AP2B1 Antibody

Binding Specificity: Middle Region

Sequence: SETQELVQQV LSLATQDSDN PDLRDRGYIY WRLLSTDPVT AKEVVLSEKP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against AP2B1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AP2B1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 951 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-AP2B1 antibody (ABIN2785454)

Schmid, Ford, Burtey, Praefcke, Peak-Chew, Mills, Benmerah, McMahon: "Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly." in: PLoS biology, Vol. 4, Issue 9, pp. e262, (2006) (PubMed).

Target Details for AP2B1

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Alternative Name: AP2B1

Background: AP2B1is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. AP2B1 is found on the cytoplasmic face of coated vesicles in the plasma membrane.The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: ADTB2, AP105B, AP2-BETA, CLAPB1, DKFZp781K0743
Protein Interaction Partner: NEU4, XRCC6BP1, THAP1, U2AF1, KPNA2, SLC25A6, AFF4, TXN2, PIP5K1C, RAPGEF3, AP1M2, SUMO2, GTF2I, UBC, EGFR, SUMO1, NEDD8, EPHA2, RPA3, RPA2, RPA1, SEC24C, NUSAP1, EPN1, XPO7, PLOD2, LNPEP, GRB2, LDLRAP1, AP2M1, UBD, RNF11, MMS19, ITGA4, FN1, ERBB2, DAB2,
Protein Size: 951

Molecular Weight: 105 kDa

Gene ID: 163

NCBI Accession: NM_001030006, NP_001025177

UniProt: P63010

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation