QTRT1 antibody (Catalytic Subunit 1) (Biotin)

Catalog No. ABIN7166919

The Rabbit Polyclonal anti-QTRT1 antibody has been validated for ELISA. It is suitable to detect QTRT1 in samples from Human.

Quick Overview for QTRT1 antibody (Catalytic Subunit 1) (Biotin) (ABIN7166919)

Target: QTRT1 (Queuine tRNA-Ribosyltransferase 1 (QTRT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This QTRT1 antibody is conjugated to Biotin

Application: ELISA

Product Details anti-QTRT1 Antibody (Biotin)

Binding Specificity: AA 211-403, Catalytic Subunit 1

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Queuine tRNA-ribosyltransferase catalytic subunit 1 protein (211-403AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for QTRT1

Target: QTRT1 (Queuine tRNA-Ribosyltransferase 1 (QTRT1))

Alternative Name: QTRT1

Background:

Background: Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1\' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1\' of the ribose to form the product (By similarity).

Aliases: FP3235 antibody, Guanine insertion enzyme antibody, qtrt1 antibody, Queuine tRNA ribosyltransferase 1 antibody, Queuine tRNA ribosyltransferase antibody, Queuine tRNA-ribosyltransferase antibody, TGT antibody, TGT, 43 KD subunit antibody, TGT, catalytic subunit antibody, TGT_HUMAN antibody, TGUT antibody, tRNA guanine transglycosylase antibody, tRNA-guanine transglycosylase antibody

UniProt: Q9BXR0

Pathways: Ribonucleoside Biosynthetic Process