NEK2 antibody (AA 1-135)

Catalog No. ABIN7169100

The Rabbit Polyclonal anti-NEK2 antibody has been validated for ELISA and IHC. It is suitable to detect NEK2 in samples from Human.

Quick Overview for NEK2 antibody (AA 1-135) (ABIN7169100)

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-NEK2 Antibody

Binding Specificity: AA 1-135

Cross-Reactivity: Human

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Serine/threonine-protein kinase Nek2 protein (1-135AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Alternative Name: NEK2

Background:

Background: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC, SGOL1, NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.

Aliases: HSPK 21 antibody, HsPK21 antibody, NEK 2 antibody, NEK2 antibody, NEK2_HUMAN antibody, NEK2A antibody, Never in mitosis A-related kinase 2 antibody, NIMA (never in mitosis gene a) related kinase 2 antibody, NimA like protein kinase 1 antibody, NIMA related kinase 2 antibody, NimA related protein kinase 2 antibody, NimA-like protein kinase 1 antibody, NimA-related protein kinase 2 antibody, NLK 1 antibody, NLK1 antibody, Serine/threonine-protein kinase Nek2 antibody

UniProt: P51955

Pathways: M Phase