SRPK1 antibody (AA 290-480)

Catalog No. ABIN7170676

This anti-SRPK1 antibody is a Rabbit Polyclonal antibody detecting SRPK1 in ELISA and IHC. Suitable for Human.

Quick Overview for SRPK1 antibody (AA 290-480) (ABIN7170676)

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRPK1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-SRPK1 Antibody

Binding Specificity: AA 290-480

Cross-Reactivity: Human

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human SRSF protein kinase 1 protein (290-480AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for SRPK1

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Alternative Name: SRPK1

Background:

Background: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation.

Aliases: Serine/arginine rich protein specific kinase 1 antibody, Serine/arginine rich splicing factor kinase 1 antibody, Serine/arginine-rich protein-specific kinase 1 antibody, Serine/threonine protein kinase SRPK1 antibody, Serine/threonine-protein kinase SRPK1 antibody, SFRS protein kinase 1 antibody, SFRSK1 antibody, SR protein kinase 1 antibody, SR protein specific kinase 1 antibody, SR-protein-specific kinase 1 antibody, SRPK1 antibody, SRPK1_HUMAN antibody

UniProt: Q96SB4

Pathways: Toll-Like Receptors Cascades