SdeA (AA 19-268) antibody

Catalog No. ABIN7176790

Product Details

Target: SdeA

Binding Specificity: AA 19-268

Reactivity: Legionella pneumophila

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Cross-Reactivity: Legionella pneumophila

Purification: >95%, Protein G purified

Immunogen: Recombinant Legionella pneumophila subsp Ubiquitinating/deubiquitinating enzyme SdeA protein (19-268AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details

Target: SdeA

Background:

Background: Secreted effector that interferes with the host cell ubiquitin pathway and is required for intracellular bacterial replication. Catalyzes the ubiquitination of several mammalian Rab proteins (Rab33b, Rab1, Rab6a and Rab30) during L.pneumophila infection, without engaging the standard cellular enzyme cascade (E1 and E2). Transfers an ADP-ribose moiety from NAD to the \'Arg-42\' residue of ubiquitin in a reaction that releases nicotinamide. The modified ubiquitin is subsequently transferred to the substrate protein through an unknown mechanism that results in the release of AMP. Cannot ubiquitinate the endosomal Rab5 or the cytoskeletal small GTPase Rac1 (PubMed:27049943). Also acts as a deubiquitinase (DUB), catalyzing the cleavage of three of the most abundant polyubiquitin chains (\'Lys-11\', \'Lys-48\' and \'Lys-63\') with a distinct preference for \'Lys-63\' linkages, is thus able to efficiently remove \'Lys-63\'-linked polyubiquitin chains from the phagosomal surface. Is also able to remove NEDD8 from neddylated proteins, but is unable to recognize SUMO. The DUB activity of SdeA is important for regulating the dynamics of ubiquitin association with the bacterial phagosome, but is dispensable for its role in intracellular bacterial replication (PubMed:26598703).

Aliases: sdeA antibody, lpg2157 antibody, Ubiquitinating/deubiquitinating enzyme SdeA antibody, Effector protein SdeA) [Includes: Deubiquitinase antibody, DUB antibody, EC 3.4.22.- antibody, Deneddylase antibody, Deubiquitinating enzyme), Ubiquitin transferase antibody, EC 2.3.2.-), Mono-ADP-ribosyltransferase antibody, mART antibody, EC 2.4.2.31)] antibody

UniProt: Q5ZTK4