Gag (AA 216-478) antibody (Biotin)

Catalog No. ABIN7176874

Product Details

Target: Gag

Binding Specificity: AA 216-478

Reactivity: Moloney Murine Leukemia Virus (MoMuLV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA

Cross-Reactivity: Moloney Murine Leukemia Virus (MoMuLV)

Purification: >95%, Protein G purified

Immunogen: Recombinant Moloney murine leukemia virus Gag polyprotein protein (216-478AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details

Target: Gag

Target Type: Viral Protein

Background:

Background: Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release.Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex.Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5\\\'-end of the genome. This binding is dependent on genome dimerization.

Aliases: gag antibody, Gag polyprotein antibody, Pr65gag antibody, Core polyprotein) [Cleaved into: Matrix protein p15 antibody, MA), RNA-binding phosphoprotein p12 antibody, pp12), Capsid protein p30 antibody, CA), Nucleocapsid protein p10-Gag antibody, NC-gag)] antibody

UniProt: P03332