CDH6 antibody (AA 281-380)

Catalog No. ABIN715286

The Rabbit Polyclonal anti-CDH6 antibody has been validated for WB, ELISA and FACS and has been independently validated. It is suitable to detect CDH6 in samples from Human.

Quick Overview for CDH6 antibody (AA 281-380) (ABIN715286)

Target: CDH6 (K-Cadherin (CDH6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDH6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CDH6 Antibody

Binding Specificity: AA 281-380

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human K Cadherin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100

Restrictions: For Research Use only

Independent Validation (FACS)

Validation #029743 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029743

Date: 07/01/2014

Lot Number: 130823

Method validated: Flow Cytometry

Positive Control: 786-O cells and [MCF-7 cells] (http://www.nature.com/bjc/journal/v90/n5/fig_tab/6601640f10.html#figure-title)

Notes: As expected, strong signal was observed in the cells stained with anti-K-Cadherin (CDH6) antibody. A small amount of non-specific staining was observed from the isotype and secondary antibody only negative controls compared with unstained cells.

Validation Images

Figure 1. Histogram of 786-O cells stained with anti-K-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

Figure 2. Histogram of MCF-7 cells stained with anti-K-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

Full Methods

Primary Antibody:

• Antigen: K-Cadherin (CDH6) antibody
• Catalog number: ABIN715286
• Lot number: 130823

Secondary Antibody:

• Antibody: Goat anti-rabbit IgG-Alexa 647

Full Protocol:

• Positive control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
• Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - 1 µg primary antibody - 1 µg isotype control antibody
• Cells were incubated for 30 min on ice.
• Labeled cells were washed twice in PBS containing 0.5% BSA.
• Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
• Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
• Labeled cells were washed twice in PBS containing 0.5% BSA.
• Propidium Iodide (PI) was added to discern live cells from dead cells.
• Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Experimental Notes: - The data displayed is gated on PI negative cells.

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CDH6

Target: CDH6 (K-Cadherin (CDH6))

Alternative Name: K Cadherin

Background:

Synonyms: CAD6, KCAD, Cadherin-6, Kidney cadherin, K-cadherin, CDH6

Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

Gene ID: 1004

UniProt: P55285

Pathways: Cell-Cell Junction Organization