YWHAB/YWHAZ antibody (pSer184, pSer186)

Catalog No. ABIN7224962

The Rabbit Polyclonal anti-YWHAB/YWHAZ antibody has been validated for WB and ELISA. It is suitable to detect YWHAB/YWHAZ in samples from Human, Mouse and Rat.

Quick Overview for YWHAB/YWHAZ antibody (pSer184, pSer186) (ABIN7224962)

Target: YWHAB/YWHAZ (14-3-3 beta/zeta (YWHAB/YWHAZ))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAB/YWHAZ antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-YWHAB/YWHAZ Antibody

Binding Specificity: pSer184, pSer186

Purpose: 14-3-3 β/ζ (phospho Ser184/186) Polyclonal Antibody

Specificity: Phospho-14-3-3 β/ζ (S184/186) Polyclonal Antibody detects endogenous levels of 14-3-3 β/ζ protein only when phosphorylated at S184/186.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human 14-3-3 beta/zeta Phospho-Ser184/186

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for YWHAB/YWHAZ

Target: YWHAB/YWHAZ (14-3-3 beta/zeta (YWHAB/YWHAZ))

Alternative Name: 14-3-3 beta/zeta

Background: Rabbit Anti-14-3-3 β/ζ (phospho Ser184/186) Polyclonal Antibody,YWHAZ, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB, 14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1,YWHAZ (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta) product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for YWHAZ.,14-3-3 protein beta

Gene ID: 7534, 7529