YWHAE antibody (AA 180-260)

Catalog No. ABIN7213368

This Rabbit Polyclonal antibody specifically detects YWHAE in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for YWHAE antibody (AA 180-260) (ABIN7213368)

Target: YWHAE (14-3-3 epsilon (YWHAE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-YWHAE Antibody

Binding Specificity: AA 180-260

Purpose: 14-3-3 ε Polyclonal Antibody

Specificity: 14-3-3 ε Polyclonal Antibody detects endogenous levels of 14-3-3 ε protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human 14-3-3 epsilon at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for YWHAE

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: 14-3-3 epsilon

Background: Rabbit Anti-14-3-3 ε Polyclonal Antibody,YWHAE, 14-3-3 protein epsilon, 14-3-3E,Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for YWHAE.,14-3-3 protein epsilon

Gene ID: 7531

UniProt: P62258

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase