14-3-3 gamma antibody (AA 30-110)

Catalog No. ABIN7224954

This Rabbit Polyclonal antibody specifically detects 14-3-3 gamma in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for 14-3-3 gamma antibody (AA 30-110) (ABIN7224954)

Target: 14-3-3 gamma (YWHAG1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-14-3-3 gamma Antibody

Binding Specificity: AA 30-110

Purpose: 14-3-3 γ Polyclonal Antibody

Specificity: 14-3-3 γ Polyclonal Antibody detects endogenous levels of 14-3-3 γ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human 14-3-3 gamma at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for 14-3-3 gamma

Target: 14-3-3 gamma (YWHAG1)

Alternative Name: 14-3-3 gamma

Background: Rabbit Anti-14-3-3 γ Polyclonal Antibody,YWHAG, 14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1,YWHAG (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma) product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.,14-3-3 protein gamma

Gene ID: 7532

UniProt: P61981

Pathways: Myometrial Relaxation and Contraction, M Phase