Acetyl-CoA Carboxylase alpha antibody

Catalog No. ABIN7213446

The Rabbit Polyclonal anti-Acetyl-CoA Carboxylase alpha antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect Acetyl-CoA Carboxylase alpha in samples from Human, Mouse and Rat.

Quick Overview for Acetyl-CoA Carboxylase alpha antibody (ABIN7213446)

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetyl-CoA Carboxylase alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Acetyl-CoA Carboxylase alpha Antibody

Purpose: ACCα Polyclonal Antibody

Specificity: ACCα Polyclonal Antibody detects endogenous levels of ACCα protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human ACCalpha around the non-phosphorylation site of S80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Acetyl-CoA Carboxylase alpha

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Alternative Name: ACCalpha

Background: Rabbit Anti-ACCα Polyclonal Antibody,ACACA, ACAC, ACC1, ACCA, Acetyl-CoA carboxylase 1, ACC1, ACC-alpha,Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for ACACA.,Acetyl-CoA carboxylase 1

Gene ID: 31

UniProt: Q13085

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effect