Aconitase 1 antibody (pSer711)

Catalog No. ABIN7222760

This anti-Aconitase 1 antibody is a Rabbit Polyclonal antibody detecting Aconitase 1 in WB, ELISA and IHC (p). Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for Aconitase 1 antibody (pSer711) (ABIN7222760)

Target: Aconitase 1 (ACO1)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Aconitase 1 Antibody

Binding Specificity: pSer711

Purpose: IRP-1 (phospho Ser711) Polyclonal Antibody

Specificity: Phospho-IRP-1 (S711) Polyclonal Antibody detects endogenous levels of IRP-1 protein only when phosphorylated at S711.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human IRP-1 Phospho-Ser711

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: IRP-1

Background: Rabbit Anti-IRP-1 (phospho Ser711) Polyclonal Antibody,ACO1, IREB1, Cytoplasmic aconitate hydratase, Aconitase, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, IRP1, Iron-responsive element-binding protein 1, IRE-BP 1,The protein encoded by ACO1 is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The protein encoded by ACO1 has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants.,Cytoplasmic aconitate hydratase

Molecular Weight: observerd band 85kDa

Gene ID: 48

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis