Ataxin 1 antibody (pSer776)

Catalog No. ABIN7224158

This Rabbit Polyclonal antibody specifically detects Ataxin 1 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Mouse and Human.

Quick Overview for Ataxin 1 antibody (pSer776) (ABIN7224158)

Target: Ataxin 1 (ATXN1)

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ataxin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Ataxin 1 Antibody

Binding Specificity: pSer776

Purpose: Ataxin-1 (phospho Ser776) Polyclonal Antibody

Specificity: Phospho-Ataxin-1 (S776) Polyclonal Antibody detects endogenous levels of Ataxin-1 protein only when phosphorylated at S776.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Ataxin-1 Phospho-Ser776

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Ataxin 1

Target: Ataxin 1 (ATXN1)

Alternative Name: Ataxin-1

Background: Rabbit Anti-Ataxin-1 (phospho Ser776) Polyclonal Antibody,ATXN1, ATX1, SCA1, Ataxin-1, Spinocerebellar ataxia type 1 protein,The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: aDCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the pure cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 40-83 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). At least two transcript variants encoding the same protein have been found for ATXN1 (ataxin 1).,Ataxin-1

Gene ID: 6310

UniProt: P54253

Pathways: Synaptic Membrane