BIK antibody (pSer35)

Catalog No. ABIN7224186

This Rabbit Polyclonal antibody specifically detects BIK in ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for BIK antibody (pSer35) (ABIN7224186)

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIK antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BIK Antibody

Binding Specificity: pSer35

Purpose: NBK (phospho Ser35) Polyclonal Antibody

Specificity: Phospho-NBK (S35) Polyclonal Antibody detects endogenous levels of NBK protein only when phosphorylated at S35.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human NBK Phospho-Ser35

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BIK

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Alternative Name: NBK

Background: Rabbit Anti-NBK (phospho Ser35) Polyclonal Antibody,BIK, NBK, Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4,The protein encoded by BIK (BCL2 interacting killer) shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.,Bcl-2-interacting killer

Gene ID: 638

UniProt: Q13323

Pathways: Positive Regulation of Endopeptidase Activity