BUB1B antibody (AA 310-390)

Catalog No. ABIN7213866

This anti-BUB1B antibody is a Rabbit Polyclonal antibody detecting BUB1B in WB and ELISA. Suitable for Human and Mouse.

Quick Overview for BUB1B antibody (AA 310-390) (ABIN7213866)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BUB1B Antibody

Binding Specificity: AA 310-390

Purpose: BubR1 Polyclonal Antibody

Specificity: BubR1 Polyclonal Antibody detects endogenous levels of BubR1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human BubR1 at AA range: 310-390

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BubR1

Background: Rabbit Anti-BubR1 Polyclonal Antibody,BUB1B, BUBR1, MAD3L, SSK1, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, MAD3/BUB1-related protein kinase, hBUBR1, Mitotic checkpoint kinase MAD3L, Protein SSK1,BUB1B encodes a kinase involved in spindle checkpoint function. The protein (BUB1 mitotic checkpoint serine/threonine kinase B)has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.,Mitotic checkpoint serine threonine-protein kinase BUB1 beta

Molecular Weight: observerd band 130kDa

Gene ID: 701

UniProt: O60566