CAD antibody

Catalog No. ABIN7225038

The Rabbit Polyclonal anti-CAD antibody has been validated for ELISA and IHC (p). It is suitable to detect CAD in samples from Human and Mouse.

Quick Overview for CAD antibody (ABIN7225038)

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAD antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CAD Antibody

Purpose: CAD Polyclonal Antibody

Specificity: CAD Polyclonal Antibody detects endogenous levels of CAD protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human CAD around the non-phosphorylation site of T456

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CAD

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Alternative Name: CAD

Background: Rabbit Anti-CAD Polyclonal Antibody,CAD, CAD protein,The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.,CAD protein

Molecular Weight: observerd band 250kDa

Gene ID: 790

UniProt: P27708

Pathways: Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process