CEBPB antibody (pThr235)

Catalog No. ABIN7212896

The Rabbit Polyclonal anti-CEBPB antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect CEBPB in samples from Human, Mouse and Rat.

Quick Overview for CEBPB antibody (pThr235) (ABIN7212896)

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CEBPB Antibody

Binding Specificity: pThr235

Purpose: C/EBP β (phospho Thr235) Polyclonal Antibody

Specificity: Phospho-C/EBP β (T235) Polyclonal Antibody detects endogenous levels of C/EBP β protein only when phosphorylated at T235.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human C/EBP beta Phospho-Thr235

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CEBPB

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Alternative Name: C/EBP beta

Background: Rabbit Anti-C/EBP β (phospho Thr235) Polyclonal Antibody,CEBPB, LAP, TCF5, PP9092, CCAAT/enhancer-binding protein beta, C/EBP beta, Liver activator protein, Nuclear factor NF-IL6, Transcription factor 5, TCF-5,This intronless gene (CEBPB) encodes a transcription factor (CCAAT/enhancer binding protein beta) that contains a basic leucine zipper (bZIP) domain. CCAAT/enhancer binding protein beta functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.,CCAAT

Molecular Weight: observerd band 36kDa

Gene ID: 1051

UniProt: P17676

Pathways: Interferon-gamma Pathway, Autophagy, Brown Fat Cell Differentiation