CEBPE antibody (pThr74)

Catalog No. ABIN7219040

This Rabbit Polyclonal antibody specifically detects CEBPE in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CEBPE antibody (pThr74) (ABIN7219040)

Target: CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CEBPE Antibody

Binding Specificity: pThr74

Purpose: C/EBP ε (phospho Thr74) Polyclonal Antibody

Specificity: Phospho-C/EBP ε (T74) Polyclonal Antibody detects endogenous levels of C/EBP ε protein only when phosphorylated at T74.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human C/EBP epsilon Phospho-Thr74

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CEBPE

Target: CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

Alternative Name: C/EBP epsilon

Background: Rabbit Anti-C/EBP ε (phospho Thr74) Polyclonal Antibody,CEBPE, CCAAT/enhancer-binding protein epsilon, C/EBP epsilon,The CCAAT/enhancer binding protein epsilon encoded by CEBPE is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined.,CCAAT enhancer-binding protein epsilon

Molecular Weight: observerd band 34kDa

Gene ID: 1053

UniProt: Q15744

Pathways: Cellular Response to Molecule of Bacterial Origin