CD53 antibody (Internal Region)

Catalog No. ABIN7218686

The Rabbit Polyclonal anti-CD53 antibody has been validated for WB and ELISA. It is suitable to detect CD53 in samples from Human, Mouse and Rat.

Quick Overview for CD53 antibody (Internal Region) (ABIN7218686)

Target: CD53

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD53 Antibody

Binding Specificity: Internal Region

Purpose: CD53 Polyclonal Antibody

Specificity: CD53 Polyclonal Antibody detects endogenous levels of CD53 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human CD53

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CD53

Target: CD53

Alternative Name: CD53

Background: Rabbit Anti-CD53 Polyclonal Antibody,CD53, MOX44, TSPAN25, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25, CD53,CD53 Molecule encoded by CD53 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants.,Leukocyte surface antigen CD53

Molecular Weight: observerd band 24kDa

Gene ID: 963

UniProt: P19397