CDCA3 antibody (AA 190-270)

Catalog No. ABIN7214118

This anti-CDCA3 antibody is a Rabbit Polyclonal antibody detecting CDCA3 in WB, ELISA, IF and IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for CDCA3 antibody (AA 190-270) (ABIN7214118)

Target: CDCA3 (Cell Division Cycle Associated 3 (CDCA3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDCA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDCA3 Antibody

Binding Specificity: AA 190-270

Purpose: CdcA3 Polyclonal Antibody

Specificity: CdcA3 Polyclonal Antibody detects endogenous levels of CdcA3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human CdcA3 at AA range: 190-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CDCA3

Target: CDCA3 (Cell Division Cycle Associated 3 (CDCA3))

Alternative Name: CdcA3

Background: Rabbit Anti-CdcA3 Polyclonal Antibody,CDCA3, C8, GRCC8, TOME1, Cell division cycle-associated protein 3, Gene-rich cluster protein C8, Trigger of mitotic entry protein 1, TOME-1,Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. CdcA3 (Cell division cycle-associated protein 3), also known as TOME1 (Trigger of mitotic entry protein 1) and GRCC8 (Gene-rich cluster protein C8), is a 268 amino acid cytoplasmic protein that is required for entry into mitosis. By participating with E3 ligase complexes, CdcA3 is involved in ubiquination and degradation of the mitosis-inhibitory kinase Wee 1, leading to the dephosphorylation of Cdc2 in the late G2 phase. CdcA3 itself is targeted for destruction by anaphase promoting complex (APC) in the G1 phase of the cell cycle, then allowing for Wee 1 accumulation during interphase.,Cell division cycle-associated protein 3

Molecular Weight: observerd band 28kDa

Gene ID: 83461

UniProt: Q99618