Factor VIII antibody (AA 2130-2210)

Catalog No. ABIN7214840

This Rabbit Polyclonal antibody specifically detects Factor VIII in WB, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Factor VIII antibody (AA 2130-2210) (ABIN7214840)

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Factor VIII antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Factor VIII Antibody

Binding Specificity: AA 2130-2210

Purpose: Factor VIII Polyclonal Antibody

Specificity: Factor VIII Polyclonal Antibody detects endogenous levels of Factor VIII protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Factor VIII at AA range: 2130-2210

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Factor VIII

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Alternative Name: Factor VIII

Background: Rabbit Anti-Factor VIII Polyclonal Antibody,F8, F8C, Coagulation factor VIII, Antihemophilic factor, AHF, Procoagulant component,F8 encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation, factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. F8 produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. DefectsF8 results in hemophilia A, a common recessive X-linked coagulation disorder.,Coagulation factor VIII

Molecular Weight: observerd band 300kDa

Gene ID: 2157

UniProt: P00451