DDX3X antibody

Catalog No. ABIN7220008

This Rabbit Polyclonal antibody specifically detects DDX3X in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for DDX3X antibody (ABIN7220008)

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX3X antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDX3X Antibody

Purpose: DDX3 Polyclonal Antibody

Specificity: DDX3 Polyclonal Antibody detects endogenous levels of DDX3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human DDX3 around the non-phosphorylation site of Y69

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for DDX3X

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Alternative Name: DDX3

Background: Rabbit Anti-DDX3 Polyclonal Antibody,DDX3X, DBX, DDX3, ATP-dependent RNA helicase DDX3X, DEAD box protein 3, X-chromosomal, DEAD box, X isoform, Helicase-like protein 2, HLP2, DDX3Y, DBY, ATP-dependent RNA helicase DDX3Y, DEAD box protein 3, Y-chromosomal,DEAD-box helicase 3, X-linked encoded by DDX3X is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.,ATP-dependent RNA helicase DDX3X

Molecular Weight: observerd band 73kDa

Gene ID: 1654, 8653

Pathways: Ribonucleoprotein Complex Subunit Organization, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Ribosome Assembly