DOK3 antibody (AA 70-150)

Catalog No. ABIN7214606

The Rabbit Polyclonal anti-DOK3 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect DOK3 in samples from Human and Mouse.

Quick Overview for DOK3 antibody (AA 70-150) (ABIN7214606)

Target: DOK3 (Docking Protein 3 (DOK3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DOK3 Antibody

Binding Specificity: AA 70-150

Purpose: Dok-3 Polyclonal Antibody

Specificity: Dok-3 Polyclonal Antibody detects endogenous levels of Dok-3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Dok-3 at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for DOK3

Target: DOK3 (Docking Protein 3 (DOK3))

Alternative Name: Dok-3

Background: Rabbit Anti-Dok-3 Polyclonal Antibody,DOK3, Docking protein 3, Downstream of tyrosine kinase 3,Dok-1, Dok-2 and Dok-3 are members of a class of "docking" proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Dok-2 (also designated p56 Dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl. Dok-3 is an adapter involved in the recruitment of inhibitory molecules and is highly expressed in B cells and macrophages. Immunoreceptor-mediated cellular activation induces tyrosine phosphorylation of Dok-3. Upon phosphorylation, Dok-3 binds to 5' inositol phosphatase SHIP and the protein tyrosine kinase Csk. Dok-3 may play a significant role in the negative regulation of immunoreceptor signaling in hemopoietic cells.,Docking protein 3

Molecular Weight: observerd band 58kDa

Gene ID: 79930

UniProt: Q7L591