DUSP1 antibody (pSer359)

Catalog No. ABIN7220096

The Rabbit Polyclonal anti-DUSP1 antibody has been validated for ELISA and IHC (p). It is suitable to detect DUSP1 in samples from Human.

Quick Overview for DUSP1 antibody (pSer359) (ABIN7220096)

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DUSP1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DUSP1 Antibody

Binding Specificity: pSer359

Purpose: MKP-1 (phospho Ser359) Polyclonal Antibody

Specificity: Phospho-MKP-1 (S359) Polyclonal Antibody detects endogenous levels of MKP-1 protein only when phosphorylated at S359.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human MKP-1 Phospho-Ser359

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for DUSP1

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Alternative Name: MKP-1

Background: Rabbit Anti-MKP-1 (phospho Ser359) Polyclonal Antibody,DUSP1, CL100, MKP1, PTPN10, VH1, Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, Mitogen-activated protein kinase phosphatase 1, MAP kinase phosphatase 1, MKP-1, Protein-tyrosine phosphatase CL100,The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.,Dual specificity protein phosphatase 1

Gene ID: 1843

UniProt: P28562