EPS15 antibody (pTyr849)

Catalog No. ABIN7220316

This anti-EPS15 antibody is a Rabbit Polyclonal antibody detecting EPS15 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for EPS15 antibody (pTyr849) (ABIN7220316)

Target: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPS15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EPS15 Antibody

Binding Specificity: pTyr849

Purpose: Eps15 (phospho Tyr849) Polyclonal Antibody

Specificity: Phospho-Eps15 (Y849) Polyclonal Antibody detects endogenous levels of Eps15 protein only when phosphorylated at Y849.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Eps15 Phospho-Tyr849

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for EPS15

Target: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Alternative Name: Eps15

Background: Rabbit Anti-Eps15 (phospho Tyr849) Polyclonal Antibody,EPS15, AF1P, Epidermal growth factor receptor substrate 15, Protein Eps15, Protein AF-1p,EPS15 encodes epidermal growth factor receptor pathway substrate 15 that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, EPS15 is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.,Epidermal growth factor receptor substrate 15

Molecular Weight: observerd band 100kDa

Gene ID: 2060

UniProt: P42566

Pathways: EGFR Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, EGFR Downregulation