Dematin antibody

Catalog No. ABIN7214548

This Rabbit Polyclonal antibody specifically detects Dematin in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Dematin antibody (ABIN7214548)

Target: Dematin (EPB49) (erythrocyte Membrane Protein Band 4.9 (Dematin) (EPB49))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dematin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Dematin Antibody

Purpose: Dematin Polyclonal Antibody

Specificity: Dematin Polyclonal Antibody detects endogenous levels of Dematin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Dematin around the non-phosphorylation site of S403

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Dematin

Target: Dematin (EPB49) (erythrocyte Membrane Protein Band 4.9 (Dematin) (EPB49))

Alternative Name: Dematin

Background: Rabbit Anti-Dematin Polyclonal Antibody,EPB49, DMT, Dematin, Erythrocyte membrane protein band 4.9,Dematin actin binding protein encoded by DMTN is an actin binding and bundling protein that plays a structural role in erythrocytes, by stabilizing and attaching the spectrin/actin cytoskeleton to the erythrocyte membrane in a phosphorylation-dependent manner. This protein contains a core domain in the N-terminus, and a headpiece domain in the C-terminus that binds F-actin. When purified from erythrocytes, this protein exists as a trimer composed of two 48 kDa polypeptides and a 52 kDa polypeptide. The different subunits arise from alternative splicing in the 3' coding region, where the headpiece domain is located. Disruption of this gene has been correlated with the autosomal dominant Marie Unna hereditary hypotrichosis disease, while loss of heterozygosity of DMTN is thought to play a role in prostate cancer progression. Alternative splicing results in multiple transcript variants encoding different isoforms.,Dematin

Molecular Weight: observerd band 50kDa

Gene ID: 2039

UniProt: Q08495

Pathways: Regulation of Actin Filament Polymerization