eEF1A1 antibody (AA 270-350)

Catalog No. ABIN7224836

The Rabbit Polyclonal anti-eEF1A1 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect eEF1A1 in samples from Human, Mouse and Rat.

Quick Overview for eEF1A1 antibody (AA 270-350) (ABIN7224836)

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eEF1A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-eEF1A1 Antibody

Binding Specificity: AA 270-350

Purpose: EF-Tu Polyclonal Antibody

Specificity: EF-Tu Polyclonal Antibody detects endogenous levels of EF-Tu protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human EF-Tu at AA range: 270-350

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for eEF1A1

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Alternative Name: EF-Tu

Background: Rabbit Anti-EF-Tu Polyclonal Antibody,TUFM, Elongation factor Tu, mitochondrial, EF-Tu, P43,TUFM (Tu translation elongation factor, mitochondrial) encodes a protein which participates in protein translation in mitochondria. Mutations in TUFM have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17.,Elongation factor Tu mitochondrial

Molecular Weight: observerd band 50kDa

Gene ID: 7284

UniProt: P49411