EIF4G1 antibody (pSer1148)

Catalog No. ABIN7220204

This anti-EIF4G1 antibody is a Rabbit Polyclonal antibody detecting EIF4G1 in ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for EIF4G1 antibody (pSer1148) (ABIN7220204)

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4G1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF4G1 Antibody

Binding Specificity: pSer1148

Purpose: eIF4G (phospho Ser1148) Polyclonal Antibody

Specificity: Phospho-eIF4G (S1148) Polyclonal Antibody detects endogenous levels of eIF4G protein only when phosphorylated at S1148.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human eIF4G Phospho-Ser1148

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for EIF4G1

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Alternative Name: eIF4G

Background: Rabbit Anti-eIF4G (phospho Ser1148) Polyclonal Antibody,EIF4G1, EIF4F, EIF4G, EIF4GI, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220,The eukaryotic translation initiation factor 4 gamma 1 encoded by EIF4G1 is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.,Eukaryotic translation initiation factor 4 gamma 1

Gene ID: 1981

UniProt: Q04637