FZD6 antibody (AA 90-170)

Catalog No. ABIN7225244

This Rabbit Polyclonal antibody specifically detects FZD6 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for FZD6 antibody (AA 90-170) (ABIN7225244)

Target: FZD6 (Frizzled Family Receptor 6 (FZD6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FZD6 Antibody

Binding Specificity: AA 90-170

Purpose: Frizzled-6 Polyclonal Antibody

Specificity: Frizzled-6 Polyclonal Antibody detects endogenous levels of Frizzled-6 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Frizzled-6 at AA range: 90-170

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for FZD6

Target: FZD6 (Frizzled Family Receptor 6 (FZD6))

Alternative Name: Frizzled-6

Background: Rabbit Anti-Frizzled-6 Polyclonal Antibody,FZD6, Frizzled-6, Fz-6, hFz6,FZD6 (frizzled class receptor 6) represents a member of the frizzled gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.,Frizzled-6

Molecular Weight: observerd band 79kDa

Gene ID: 8323

UniProt: O60353

Pathways: WNT Signaling, Tube Formation