GABARAP antibody

Catalog No. ABIN7226848

The Rabbit Polyclonal anti-GABARAP antibody has been validated for WB and ELISA. It is suitable to detect GABARAP in samples from Human, Mouse and Rat.

Quick Overview for GABARAP antibody (ABIN7226848)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABARAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GABARAP Antibody

Purpose: GABARAP Polyclonal Antibody

Specificity: GABARAP Polyclonal Antibody detects endogenous levels of GABARAP.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthetic Peptide of GABARAP

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Alternative Name: GABARAP

Background: Rabbit Anti-GABARAP Polyclonal Antibody,GABA(A) receptor-associated protein-like 2,GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa, GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: gABARAPL2-I, later conjugated to PE lipid as lipidated form: gABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.,GABARAP

Molecular Weight: observerd band 17kDa

Gene ID: 11345

UniProt: P60520

Pathways: Autophagy