GTF2I antibody (pTyr248)

Catalog No. ABIN7221394

This anti-GTF2I antibody is a Rabbit Polyclonal antibody detecting GTF2I in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for GTF2I antibody (pTyr248) (ABIN7221394)

Target: GTF2I (General Transcription Factor III (GTF2I))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2I antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GTF2I Antibody

Binding Specificity: pTyr248

Purpose: TFII-I (phospho Tyr248) Polyclonal Antibody

Specificity: Phospho-TFII-I (Y248) Polyclonal Antibody detects endogenous levels of TFII-I protein only when phosphorylated at Y248.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human TFII-I Phospho-Tyr248

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GTF2I

Target: GTF2I (General Transcription Factor III (GTF2I))

Alternative Name: TFII-I

Background: Rabbit Anti-TFII-I (phospho Tyr248) Polyclonal Antibody,GTF2I, BAP135, WBSCR6, General transcription factor II-I, GTFII-I, TFII-I, Bruton tyrosine kinase-associated protein 135, BAP-135, BTK-associated protein 135, SRF-Phox1-interacting protein, SPIN, Williams-Beuren syndrome chromosomal region,GTF2I encodes a phosphoprotein containing six characteristic repeat motifs. The general transcription factor IIi binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed.,General transcription factor II-I

Molecular Weight: observerd band 115kDa

Gene ID: 2969

UniProt: P78347