HMGB2 antibody (AA 100-180)

Catalog No. ABIN7215282

The Rabbit Polyclonal anti-HMGB2 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect HMGB2 in samples from Human, Mouse and Rat.

Quick Overview for HMGB2 antibody (AA 100-180) (ABIN7215282)

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HMGB2 Antibody

Binding Specificity: AA 100-180

Purpose: HMG-2 Polyclonal Antibody

Specificity: HMG-2 Polyclonal Antibody detects endogenous levels of HMG-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human HMG-2 at AA range: 100-180

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for HMGB2

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Alternative Name: HMG-2

Background: Rabbit Anti-HMG-2 Polyclonal Antibody,HMGB2, HMG2, High mobility group protein B2, High mobility group protein 2, HMG-2,HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that high mobility group protein B2 is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.,High mobility group protein B2

Molecular Weight: observerd band 27kDa

Gene ID: 3148

UniProt: P26583

Pathways: Cellular Response to Molecule of Bacterial Origin