Histone H1 antibody (acLys25)

Catalog No. ABIN7219570

The Rabbit Polyclonal anti-Histone H1 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect Histone H1 in samples from Human and Monkey.

Quick Overview for Histone H1 antibody (acLys25) (ABIN7219570)

Target: Histone H1

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H1 Antibody

Binding Specificity: acLys25

Purpose: Acetyl Histone H1 (K25) Polyclonal Antibody

Specificity: Acetyl-Histone H1 (K25) Polyclonal Antibody detects endogenous levels of Histone H1 protein only when acetylated at K25.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Histone H1 around the acetylation site of K25 at AA range: 100-180

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Histone H1

Target: Histone H1

Background: Rabbit Anti-Acetyl Histone H1 (K25) Polyclonal Antibody,H1FOO, H1OO, OSH1, Histone H1oo, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1,Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The H1 histone family member O, oocyte specific encoded is a replication-independent histone that is a member of the histone H1 family. H1FOO contains introns, unlike most histone genes. The related mouse gene is expressed only in oocytes.,Histone H1oo

Gene ID: 132243

UniProt: Q8IZA3