Histone H1 antibody (pThr17)

Catalog No. ABIN7221438

This Rabbit Polyclonal antibody specifically detects Histone H1 in IF, WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Monkey.

Quick Overview for Histone H1 antibody (pThr17) (ABIN7221438)

Target: Histone H1

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H1 Antibody

Binding Specificity: pThr17

Purpose: Histone H1 (phospho Thr17) Polyclonal Antibody

Specificity: Phospho-Histone H1 (T17) Polyclonal Antibody detects endogenous levels of Histone H1 protein only when phosphorylated at T17.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Histone H1 Phospho-Thr17

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Histone H1

Target: Histone H1

Background: Rabbit Anti-Histone H1 (phospho Thr17) Polyclonal Antibody,HIST1H1B, H1F5, Histone H1.5, Histone H1a, Histone H1b, Histone H1s-3, HIST1H1D, H1F3, Histone H1.3, Histone H1c, Histone H1s-2, HIST1H1E, H1F4, Histone H1.4, Histone H1b, Histone H1s-4,Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. HIST1H1B is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.,Histone H1.5

Molecular Weight: observerd band 31kDa

Gene ID: 3009, 3007, 3008