Histone H2A antibody (pThr121)

Catalog No. ABIN7225254

This anti-Histone H2A antibody is a Rabbit Polyclonal antibody detecting Histone H2A in WB, ELISA and IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for Histone H2A antibody (pThr121) (ABIN7225254)

Target: Histone H2A

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H2A Antibody

Binding Specificity: pThr121

Purpose: Histone H2A (phospho Thr121) Polyclonal Antibody

Specificity: Phospho-Histone H2A (T121) Polyclonal Antibody detects endogenous levels of Histone H2A protein only when phosphorylated at T121.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Histone H2A Phospho-Thr121

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Histone H2A

Target: Histone H2A

Background: Rabbit Anti-Histone H2A (phospho Thr121) Polyclonal Antibody,HIST1H2AG, H2AFP, HIST1H2AI, H2AFC, HIST1H2AK, H2AFD, HIST1H2AL, H2AFI, HIST1H2AM, H2AFN, Histone H2A type 1, H2A.1, Histone H2A/p, HIST1H2AB, H2AFM, HIST1H2AE, H2AFA, Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, HIS,Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. HIST1H2AI (histone cluster 1 H2A family member i) is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from HIST1H2AI lack polyA tails but instead contain a palindromic termination element. HIST1H2AI is found in the small histone gene cluster on chromosome 6p22-p21.3.,Histone H2A type 1-B

Gene ID: 8329, 8332, 8336