Histone H4 antibody (acLys5)

Catalog No. ABIN7212574

This anti-Histone H4 antibody is a Rabbit Polyclonal antibody detecting Histone H4 in WB, IF, ELISA and IHC (p). Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for Histone H4 antibody (acLys5) (ABIN7212574)

Target: Histone H4

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H4 Antibody

Binding Specificity: acLys5

Purpose: Histone H4 (Acetyl Lys5) Polyclonal Antibody

Specificity: Acetyl-Histone H4 (K5) Polyclonal Antibody detects endogenous levels of Histone H4 protein only when acetylated at K5.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Histone H4 around the acetylation site of K5 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Histone H4

Target: Histone H4

Background: Rabbit Anti-Histone H4 (Acetyl Lys5) Polyclonal Antibody,HIST1H4A, H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK,Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST4H4 is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from HIST4H4 lack polyA tails, instead, they contain a palindromic termination element.,Histone H4

Molecular Weight: observerd band 11kDa

Gene ID: 121504, 554313, 8294

UniProt: P62805