HTRA2 antibody (pSer142)

Catalog No. ABIN7221152

The Rabbit Polyclonal anti-HTRA2 antibody has been validated for ELISA and IHC (p). It is suitable to detect HTRA2 in samples from Human, Mouse and Rat.

Quick Overview for HTRA2 antibody (pSer142) (ABIN7221152)

Target: HTRA2 (HtrA Serine Peptidase 2 (HTRA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTRA2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HTRA2 Antibody

Binding Specificity: pSer142

Purpose: HtrA2 (phospho Ser142) Polyclonal Antibody

Specificity: Phospho-HtrA2 (S142) Polyclonal Antibody detects endogenous levels of HtrA2 protein only when phosphorylated at S142.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human HtrA2 Phospho-Ser142

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for HTRA2

Target: HTRA2 (HtrA Serine Peptidase 2 (HTRA2))

Alternative Name: HtrA2

Background: Rabbit Anti-HtrA2 (phospho Ser142) Polyclonal Antibody,HTRA2, OMI, PRSS25, Serine protease HTRA2, mitochondrial, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI,HTRA2 encodes a serine protease. The HtrA serine peptidase 2 has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.,Serine protease HTRA2 mitochondrial

Gene ID: 27429

UniProt: O43464

Pathways: Positive Regulation of Endopeptidase Activity