INPP5D antibody (pTyr1021)

Catalog No. ABIN7213170

This Rabbit Polyclonal antibody specifically detects INPP5D in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for INPP5D antibody (pTyr1021) (ABIN7213170)

Target: INPP5D (Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INPP5D antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-INPP5D Antibody

Binding Specificity: pTyr1021

Purpose: SHIP-1 (phospho Tyr1021) Polyclonal Antibody

Specificity: Phospho-SHIP-1 (Y1021) Polyclonal Antibody detects endogenous levels of SHIP-1 protein only when phosphorylated at Y1021.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human SHIP-1 Phospho-Tyr1021

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for INPP5D

Target: INPP5D (Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D))

Alternative Name: SHIP-1

Background: Rabbit Anti-SHIP-1 (phospho Tyr1021) Polyclonal Antibody,INPP5D, SHIP, SHIP1, Phosphatidylinositol 3, 4,5-trisphosphate 5-phosphatase 1, Inositol polyphosphate-5-phosphatase of 145 kDa, SIP-145, SH2 domain-containing inositol 5'-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP-1,INPP5D is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes inositol polyphosphate-5-phosphatase D with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of inositol polyphosphate-5-phosphatase D is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. Inositol polyphosphate-5-phosphatase D is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in INPP5D are associated with defects and cancers of the immune system. Alternative splicing of this gene results in multiple transcript variants.,Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1

Molecular Weight: observerd band 133kDa

Gene ID: 3635

UniProt: Q92835

Pathways: TCR Signaling, BCR Signaling, Warburg Effect