Keratin 10 antibody (AA 110-190)

Catalog No. ABIN7214476

The Rabbit Polyclonal anti-Keratin 10 antibody has been validated for WB, IF, ELISA and IHC (p). It is suitable to detect Keratin 10 in samples from Human, Mouse and Rat.

Quick Overview for Keratin 10 antibody (AA 110-190) (ABIN7214476)

Target: Keratin 10 (KRT10)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Keratin 10 Antibody

Binding Specificity: AA 110-190

Purpose: Cytokeratin 10 Polyclonal Antibody

Specificity: Cytokeratin 10 Polyclonal Antibody detects endogenous levels of Cytokeratin 10 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Cytokeratin 10 at AA range: 110-190

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Cytokeratin 10

Background: Rabbit Anti-Cytokeratin 10 Polyclonal Antibody,KRT10, KPP, Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10,KRT10 encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in KRT10 are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.,Keratin type I cytoskeletal 10

Molecular Weight: observerd band 59kDa

Gene ID: 3858

UniProt: P13645