MDH1 antibody

Catalog No. ABIN7229614

This anti-MDH1 antibody is a Rabbit Polyclonal antibody detecting MDH1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for MDH1 antibody (ABIN7229614)

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MDH1 Antibody

Purpose: MDHC Polyclonal Antibody

Specificity: The antibody detects endogenous levels of MDHC protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human MDHC protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MDH1

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Alternative Name: MDHC

Background: Rabbit Anti-MDHC Polyclonal Antibody,Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase, Diiodophenylpyruvate reductase,MDH1 encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.,MDHC

Molecular Weight: observerd band 36kDa

Gene ID: 4190

UniProt: P40925