MID1 antibody (AA 40-120)

Catalog No. ABIN7215760

The Rabbit Polyclonal anti-MID1 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect MID1 in samples from Human, Mouse and Rat.

Quick Overview for MID1 antibody (AA 40-120) (ABIN7215760)

Target: MID1 (Midline 1 (MID1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MID1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MID1 Antibody

Binding Specificity: AA 40-120

Purpose: Midline-1 Polyclonal Antibody

Specificity: Midline-1 Polyclonal Antibody detects endogenous levels of Midline-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Midline-1 at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MID1

Target: MID1 (Midline 1 (MID1))

Alternative Name: Midline-1

Background: Rabbit Anti-Midline-1 Polyclonal Antibody,MID1, FXY, RNF59, TRIM18, XPRF, Midline-1, Midin, Midline 1 RING finger protein, Putative transcription factor XPRF, RING finger protein 59, Tripartite motif-containing protein 18,Midline-1 encoded by this MID1 is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. Midline-1 is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing, however, the full-length nature of some of the variants has not been determined.,Midline-1

Molecular Weight: observerd band 75kDa

Gene ID: 4281

UniProt: O15344