MARCKS antibody (pSer158)

Catalog No. ABIN7222288

This anti-MARCKS antibody is a Rabbit Polyclonal antibody detecting MARCKS in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for MARCKS antibody (pSer158) (ABIN7222288)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MARCKS Antibody

Binding Specificity: pSer158

Purpose: MARCKS (phospho Ser158) Polyclonal Antibody

Specificity: Phospho-MARCKS (S158) Polyclonal Antibody detects endogenous levels of MARCKS protein only when phosphorylated at S158.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human MARCKS Phospho-Ser158

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS

Background: Rabbit Anti-MARCKS (phospho Ser158) Polyclonal Antibody,MARCKS, MACS, PRKCSL, Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate, 80 kDa protein, light chain, 80K-L protein, PKCSL,The protein encoded by MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.,Myristoylated alanine-rich C-kinase substrate

Molecular Weight: observerd band 35kDa

Gene ID: 4082

UniProt: P29966