PFKP antibody (AA 310-390)

Catalog No. ABIN7216518

This anti-PFKP antibody is a Rabbit Polyclonal antibody detecting PFKP in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for PFKP antibody (AA 310-390) (ABIN7216518)

Target: PFKP (phosphofructokinase, Platelet (PFKP))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PFKP Antibody

Binding Specificity: AA 310-390

Purpose: PFK-C Polyclonal Antibody

Specificity: PFK-C Polyclonal Antibody detects endogenous levels of PFK-C protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human PFK-C at AA range: 310-390

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PFKP

Target: PFKP (phosphofructokinase, Platelet (PFKP))

Alternative Name: PFK-C

Background: Rabbit Anti-PFK-C Polyclonal Antibody,PFKP, PFKF, 6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, Phosphofructo-1-kinase isozyme C, PFK-C, Phosphofructokinase 1, Phosphohexokinase,The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP: D-fructose-6-phosphate-1-phosphotransferase, EC 2. . 1). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM, MIM 610681) and liver (PFKL, MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.,6-phosphofructokinase type C

Molecular Weight: observerd band 84kDa

Gene ID: 5214

UniProt: Q01813

Pathways: Warburg Effect