PLA2G4A antibody (pSer505)

Catalog No. ABIN7223166

This anti-PLA2G4A antibody is a Rabbit Polyclonal antibody detecting PLA2G4A in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for PLA2G4A antibody (pSer505) (ABIN7223166)

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLA2G4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLA2G4A Antibody

Binding Specificity: pSer505

Purpose: cPLA2 (phospho Ser505) Polyclonal Antibody

Specificity: Phospho-cPLA2 (S505) Polyclonal Antibody detects endogenous levels of cPLA2 protein only when phosphorylated at S505.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human cPLA2 Phospho-Ser505

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PLA2G4A

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Alternative Name: cPLA2

Background: Rabbit Anti-cPLA2 (phospho Ser505) Polyclonal Antibody,PLA2G4A, CPLA2, PLA2G4, Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA,PLA2G4A (phospholipase A2 group IVA) encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants.,Cytosolic phospholipase A2

Molecular Weight: observerd band 110kDa

Gene ID: 5321

UniProt: P47712

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling