PNPT1 antibody (AA 540-620)

Catalog No. ABIN7216622

This Rabbit Polyclonal antibody specifically detects PNPT1 in WB, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for PNPT1 antibody (AA 540-620) (ABIN7216622)

Target: PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNPT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PNPT1 Antibody

Binding Specificity: AA 540-620

Purpose: PNPase Polyclonal Antibody

Specificity: PNPase Polyclonal Antibody detects endogenous levels of PNPase protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human PNPase at AA range: 540-620

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PNPT1

Target: PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))

Alternative Name: PNPase

Background: Rabbit Anti-PNPase Polyclonal Antibody,PNPT1, PNPASE, Polyribonucleotide nucleotidyltransferase 1, mitochondrial, 3'-5' RNA exonuclease OLD35, PNPase old-35, Polynucleotide phosphorylase 1, PNPase 1, Polynucleotide phosphorylase-like protein,Polyribonucleotide nucleotidyltransferase 1 encoded by PNPT1 elongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. Polyribonucleotide nucleotidyltransferase 1 is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in PNPT1 have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7.,Polyribonucleotide nucleotidyltransferase 1 mitochondrial

Molecular Weight: observerd band 85kDa

Gene ID: 87178

UniProt: Q8TCS8