RAPGEF3 antibody (AA 690-770)

Catalog No. ABIN7218978

This Rabbit Polyclonal antibody specifically detects RAPGEF3 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RAPGEF3 antibody (AA 690-770) (ABIN7218978)

Target: RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAPGEF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RAPGEF3 Antibody

Binding Specificity: AA 690-770

Purpose: Epac Polyclonal Antibody

Specificity: Epac Polyclonal Antibody detects endogenous levels of Epac protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Epac at AA range: 690-770

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for RAPGEF3

Target: RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))

Alternative Name: Epac

Background: Rabbit Anti-Epac Polyclonal Antibody,RAPGEF3, CGEF1, EPAC, EPAC1, Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, Exchange protein directly activated by cAMP 1, EPAC 1, Rap1 guanine-nucleotide-exchange factor directly activated by cAMP,RAPGEF3 (Rap Guanine Nucleotide Exchange Factor 3) is a Protein Coding gene. Among its related pathways are Platelet activation, signaling and aggregation and Signaling by GPCR. GO annotations related to this gene include guanyl-nucleotide exchange factor activity and cAMP-dependent protein kinase regulator activity. An important paralog of this gene is RASGEF1A. uanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin. TPases are a group of enzymes that catalyze hydrolysis of the gamma phosphate bond in guanine triphosphate (GTP) to form guanine diphosphate (GDP). Mg2+ ions are essential for catalytic activity. GTPases, often coupled to G proteins, are essential in signal transduction.,Rap guanine nucleotide exchange factor 3

Molecular Weight: observerd band 100kDa

Gene ID: 10411

UniProt: O95398

Pathways: cAMP Metabolic Process